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內(nèi)容及儲(chǔ)存
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1 × 500 ml bottle, Store at 2 to 8°C. Protect from light.
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描述
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Essential 6? Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Thomson and published as "E8." Essential 6? Medium has been proven to support somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune? (Sendai virus). In addition, Essential 6? Medium can be used as a base for the formulation of custom media for the culture of PSCs.
Essential 6? Medium enables:?
??Reprogramming—does not contain TGFΒ, which has a negative effect on reprogramming efficiency
??Differentiation—does not contain bFGF, which inhibits embryoid body formation and differentiation
??Flexibility—provides a flexible format where the levels of TGFΒ and bFGF can be adjusted and additional components can be added to match a given application
Reprogramming
Essential 6? Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (2) and CytoTune? (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.?
Differentiation?
Essential 6? Medium is a reliable and robust medium for the formation of embryoid bodies. Unlike other media used in PSC culture, Essential 6? Medium does not contain bFGF or TGFβ, which inhibits embryoid body formation and differentiation.?
Flexibility
Essential 6? Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFΒ. This provides a basal medium that will ensure maximum cell health and pluripotency, while allowing levels of TGFΒ and bFGF to be adjusted and additional components to be added to match a given application.?