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ExpiFectamine? CHO Transfection Kit, for 10 L of culture

日期: 2015-11-16
瀏覽次數(shù): 472

貨號(hào): A29130

規(guī)格

Cell Type:???Established Cell Lines, Ovarian Cells

Sample Type (Specific):??? Chemical Solution

Classification:???? Animal Origin-Free, Chemically Defined

Transfection Technique:??? Lipid-Based Transfection

Product Size:?????1 kit

Shipping Condition:?? Room Temperature

內(nèi)容及儲(chǔ)存

32 mL ExpiFectamine CHO Reagent

60 mL ExpiFectamine CHO Enhancer

3.3 L ExpiCHO Feed

Store in the dark at 2 -8°C.

描述

The ExpiFectamine? CHO Transfection Kit is a core component of the ExpiCHO? Expression System Kit (Cat. No. A29133). It is designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will power the highest possible level of protein expression from ExpiCHO-S? Cells (Cat. No. A29127) cultured in ExpiCHO? Expression Medium (Cat. No. A2910001).

The ExpiFectamine CHO Transfection Kit:
? Achieves greater than 90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
? Requires one-third to one-half less plasmid DNA than other transfection reagents while achieving superior expression performance
? Provides options for three transient expression protocols for maximal work flexibility while still fitting into the same overall workflow used by other transient expression systems, such as FreeStyle? MAX CHO and Expi293?
? Enables robust and reproducible transfection results, giving you greater confidence in your results
? Demonstrates linear scalability of transfections from culture volumes of <1 ml="" to="">10 L

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO? Expression System Kit. The transfection kit supplies the cationic ExpiFectamine? CHO Transfection Reagent, ExpiFectamine? CHO Transfection Enhancer, and ExpiCHO Feed.




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